Data Visualization
Be Cr Mn Fe
Min. :0.00000 Min. :0.0000 Min. : 0.0000 Min. : 0.000
1st Qu.:0.00000 1st Qu.:0.0000 1st Qu.: 0.5921 1st Qu.: 2.587
Median :0.00000 Median :0.0000 Median : 2.3850 Median : 11.171
Mean :0.02552 Mean :0.1995 Mean : 55.8743 Mean : 88.809
3rd Qu.:0.00000 3rd Qu.:0.1100 3rd Qu.: 14.9455 3rd Qu.: 43.038
Max. :0.47000 Max. :3.6792 Max. :2594.0600 Max. :1370.356
Ni Cu As Se
Min. :0.00000 Min. : 0.120 Min. : 0.0000 Min. :0.00000
1st Qu.:0.08704 1st Qu.: 5.842 1st Qu.: 0.1033 1st Qu.:0.04417
Median :0.33013 Median : 32.280 Median : 0.5207 Median :0.09043
Mean :1.13954 Mean : 141.456 Mean : 11.0786 Mean :0.10569
3rd Qu.:1.21730 3rd Qu.: 139.486 3rd Qu.: 3.8104 3rd Qu.:0.14399
Max. :7.11000 Max. :3228.015 Max. :717.9056 Max. :0.40000
Cd Sb Ba Tl
Min. :0.000000 Min. :0.00000 Min. : 0.0000 Min. :0.00000
1st Qu.:0.000000 1st Qu.:0.00000 1st Qu.: 0.6428 1st Qu.:0.00000
Median :0.006647 Median :0.02133 Median : 3.8087 Median :0.00000
Mean :0.028784 Mean :0.07261 Mean : 13.4774 Mean :0.00485
3rd Qu.:0.020000 3rd Qu.:0.05047 3rd Qu.: 9.1386 3rd Qu.:0.00000
Max. :0.411806 Max. :1.49568 Max. :197.6416 Max. :0.29000
Pb U CCU
Min. : 0.01000 Min. : 0.000 Min. : 0.04017
1st Qu.: 0.09975 1st Qu.: 0.116 1st Qu.: 0.55615
Median : 0.35904 Median : 1.002 Median : 1.22744
Mean : 1.62066 Mean : 56.602 Mean : 4.67386
3rd Qu.: 1.15189 3rd Qu.: 8.181 3rd Qu.: 2.69344
Max. :20.09000 Max. :3274.370 Max. :109.91031 Plots for variation of metal concentrations across each sample
Box Plots of Metals - Identification of outliers and extreme points
Larval Activity Traces
- Substract AVG_Distance by AVG_Distance_Control to obtain the distances travelled by the larvae in the control sample, name this new variable as Control_Distance.
AVG_Distance AVG_Distance_Control Control_Distance
Min. : 1.519 Min. :-407.26 Min. :278.4
1st Qu.: 163.405 1st Qu.:-171.02 1st Qu.:290.0
Median : 261.668 Median : -70.70 Median :310.1
Mean : 369.039 Mean : 31.88 Mean :337.2
3rd Qu.: 438.187 3rd Qu.: 99.99 3rd Qu.:390.9
Max. :2569.212 Max. :2279.25 Max. :416.5 Variables in the newly created data set:
- The mean distance traveled by the larvae in each time interval for every sample - mean_D
- The mean distance traveled by larvae in each time interval relative to the control - mean_DC
- The mean distance traveled in each time interval for the control sample - mean_C
# A tibble: 10 × 6
Sample Time `Dark/Light` mean_D mean_DC mean_C
<dbl> <dbl> <chr> <dbl> <dbl> <dbl>
1 1 5 D 286. 7.29 278.
2 1 10 L 310. 20.1 290.
3 1 15 D 444. 53.6 391.
4 1 20 L 286. -23.8 310.
5 1 25 D 476. 59.9 416.
6 2 5 D 179. -99.1 278.
7 2 10 L 210. -80.0 290.
8 2 15 D 210. -181. 391.
9 2 20 L 199. -111. 310.
10 2 25 D 215. -202. 416.
Correlation matrix of concentration data and aggregate behavioral distance travelled
Created the “aggregate behavioral outcome” (ABO) variable in the metal_conc_data data set to produce the correlation matrix:
A low (0.2-0.35) positive linear correlation could be observed between ABO and:
- U
A low (0.2-0.35) negative linear correlation could be observed between ABO and:
- Ni, Cu, Cd, Ba and Pb
P-values for the correlation between metal and ABO.
Var1 Var2 p_value
1 Be ABO 0.115867212
2 Cr ABO 0.541904090
3 Mn ABO 0.434883761
4 Fe ABO 0.281244372
5 Ni ABO 0.000943512
6 Cu ABO 0.051207367
7 As ABO 0.823109698
8 Se ABO 0.182787616
9 Cd ABO 0.049504434
10 Sb ABO 0.612821775
11 Ba ABO 0.050493317
12 Tl ABO 0.130149233
13 Pb ABO 0.007649839
14 U ABO 0.048645237The metals Be, Ni, Cu, Cd, Ba, Tl, Pb and U have a P-value less than 0.15.